Part:BBa_K2165002

Violacein C gene with CUP1 promoter + ADH1 terminator

This biobrick utilizes the Cup1 promoter BBa_K2165004. Following the binding of a heavy metal to the Cup1 promoter then VioC transcription is upregulated. VioC BBa_K2165000 was used as the CDS. The biobrick ends with the ADH1 terminator BBa_K801012 for yeast that was submitted by the TU Munich team in 2012.

This biobrick was designed to characterize the codon optimized version of VioC. The codon optimized VioC should have higher translation rates than the previously made VioC, and this plasmid construct will be used to determine the change in the expression levels for the pathway. This composite part was also designed for use in the University of Washington 2016 iGEM team's chemostat-autoinjector proof of concept. For more information on the role of VioC in the violacein pathway see part BBa_K2165000.

Usage and Biology

It should be stressed that without the other main components of the Violacein pathway (VioA, VioB, and VioE, see part BBa_K726015 as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 370