DNA

Part:BBa_K2100074

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 2kturn: TP901

This is the final expression vector created from a LR between pENTR EGSH 2xk-turn and pENTR TP901. TP901 expression is suppressed by an inducible promoter, pEGSH (induced with Ponasterone A - PonA), and a RNA-based repressing system L7Ae/k-turn. The level of TP901 expression, in turn, control the expression of an output gene flanked by TP901 recognition sites (For example: pENTR attB-flipped eYFP-attP). This expression vector reduces the leaky expression (when the system is not induced with PonA) of TP901 with the L7Ae/k-turn system, decreasing the basal expression of the output gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1287
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4912
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1287
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4912
    Illegal NheI site found at 832
    Illegal NheI site found at 1098
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
    Illegal NotI site found at 2207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1287
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4912
    Illegal BglII site found at 1410
    Illegal BglII site found at 4692
    Illegal BamHI site found at 3396
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1287
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4912
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1287
    Illegal EcoRI site found at 3809
    Illegal EcoRI site found at 4912
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
    Illegal NgoMIV site found at 3560
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 709


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Categories
Parameters
None