Part:BBa_K2100049
pENTR attB-flipped eYFP-attP (TP901)
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascaded with a promoter (flanked by L4, R1 sites) using an LR reaction and cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
The flipped eYFP gene is oriented upside down, preventing translation of the gene. The flipped fluorescent gene is flanked by recognition sites of TP901 recombinase, which is oriented in a way that only allows unidirectional flip. When recombinase TP901 is present, they will bind to the recognition sites and flip the upside-down eYFP to the correct orientation, allowing the fluorescent gene to be expressed.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2163
Illegal PstI site found at 1872 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2163
Illegal NheI site found at 845
Illegal NheI site found at 1111
Illegal PstI site found at 1872 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2163
Illegal BamHI site found at 1275 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2163
Illegal PstI site found at 1872 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2163
Illegal PstI site found at 1872 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 722
| None |