Reporter

Part:BBa_K2082115

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Stop-GFP (K107*) under control of constitutive promoter

Stop GFP expression device K107*

A stop codon was introduced into BBa_E0040 at position 107 (K107*) leading to complete loss of fluorescence of this reporter.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705



Functionality was determined by cytometry, measuring single-cell fluorescence in comparison to wild type GFP in part K608010.


Figure 1: Fluorescence measurement by flow cytometry showing complete loss of fluorescence.
This reporter can be used in reversion assays, where revertants and the total cell count can be determined by flow cytometry.


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Categories
Parameters
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