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Part:BBa_K2082000

Designed by: Judith Kampa   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


Monobody: Constant regions and RFP (exchange with variable regions to create your library)

Pand-RBS-Monobody constant regions-RFP

With this fundamental Monobody framework, consisting just the constant regions of a Monobody, we provide a construct to build Monobody libraries or specific antibody mimetics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 764
    Illegal AgeI site found at 876
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a codon optimized RBS (BBa_B0034)
  • the first constant region of a Monobody CDS
  • a codon optimized Anderson promoter (BBa_J23106)
  • a RBS (BBa_B0034)
  • RFP CDS (BBa_E1010)
  • the second constant Region of a Monobody CDS


    • For constructing the Monobody library we deciced to set the design up in a way that one can exchange RFP for the fragment containing the variable (randomized) regions.


    Figure 1: Monobody construct with RFP. Construct for the Monobody with RFP insert exchangable for variable regions.

    To get further insight into the theoretical assembly take a look here.
    If you are planning to build your own Monobody library or construct a specific binder, take a look at our hints

    By using the RFP CDS the red color of colonies is a great visual control when cloning a library. Colonies that are red represent starting plasmids and not parts of the library.


    Figure 2: Colonies of dH5alpha with plasmid BBa_K2082000 shows slightly red color.


    This device is a component of our part collection, which also includes our designed Monobody library (BBa_K2082005) and also a single example monobody ( BBa_K2082009) as a positive control for your designed Monobodies for colony PCRs, sequencings and more.

    Visit our wiki for more information about our library and the opportunity of building your own library and further about our part collection. With this collection we provide every iGEM team the unprecedented chance for easily building their own Monobodies!

    If you are interested in other binding proteins, check out our Nanobody parts for setting up a library.


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