Composite

Part:BBa_K200017

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

RBS + OtsB

Composite part containing RBS for ligation to OtsA and promoter for production of trehalose.


RBS+OtsB This is an intermediate construct comprising a RBS upstream of the second enzyme for the production of trehalose. A functional transcriptional unit will be obtained upon the ligation of a promoter, RBS and the first enzyme (OtsA) upstream of the RBS. Furthermore, to close the unit a double terminator would be required downstream of the OtsB gene.


OtsB ( BBa_K200006) is the gene coding for the enzyme trehalose phosphatase. Here it has been combined with an RBS (BBa_B0034).Both OtsA and OtsB are required for the production of trehalose, so this BioBrick should be used in combination with the OtsA gene (BBa_K200005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None