Generator

Part:BBa_K1976003

Designed by: Yannik Zobus, Simon Fürbacher, Katharina Geißler, Marietheres Kleuter, Patrick Müller, Jannik Schwitte, Thomas Wagner   Group: iGEM16_TU_Darmstadt   (2016-10-13)

mVenus regulated by a T7 promoter

This part contains a IPTG inducible strong and constitutive T7 promoter, the strong Ewlotz RBS and an E. coli optimized coding sequence of mVenus with an associated LVA degradation tag leading to a regulated expression of mVenus.




Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None