Part:BBa_K1956019
RIBOSON with plasmid control
RIBOSON with plasmid control
IIT Madras 2016's Characterization
Trigger RNA: A part of RNA molecule, which codes for RFP protein.
Switch RNA: It was designed to repress the translation process by masking the RBS placed upstream of GFP protein coding part. After interacting with the RNA molecules of RFP gene, RIBOSON would allow the RBS to open up to bind to ribosomal machinery for protein synthesis to take place.
We performed our RIBOSON experiment in E. coli BL21(DE3). We transformed our cloned device BBa_K1956019, which has RIBOSONrfp switch under J23100 constitutive promoter, upstream of GFP coding part and RFP generator under lacI+pL promoter.
We grew the cells in LB broth media + 40mM Glucose. After, 16 hrs of growth, we made two types of secondary culture: 1. Cells + LB broth + 40mM Glucose and 2. Cells + LB broth + 1mM IPTG.
Positive Control: Device K1956015, which produces GFP and RFP proteins.
Negative Control: Device K1956034, which produces lacI mRNA molecules.
Below result proves the functioning of RIBOSON device.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1844
Illegal AgeI site found at 1956 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 842
| None |