Coding

Part:BBa_K1895007

Designed by: Jake Burton   Group: iGEM16_Newcastle   (2016-10-18)


Sequence for sigma 32 transcription factor

This part is a correction to BBa_M36888 which has an illegal PstI site which makes it incompatible with BioBricks / RFC10. iGEM Teams can use this alternative sequence which contains none of the BioBrick restriction enzyme sites. Amino acid No. 202, Glysine has been mutated from CAG to CAA to remove the PstI site. The remainder of the sequence remains unchanged.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 303
    Illegal SapI.rc site found at 652


[edit]
Categories
//cds/transcriptionalregulator
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//direction/forward
Parameters
familySigma 70 sigma factors
ligandsDNA Promoter Region
uniprotP0AGB3 (most common strain)