Part:BBa_M36888

Sequence for sigma 32 transcription factor

The rpoH gene codes for sigma 32 (alternatively sigma H, RpoH) transcription factor, a component of RNA polymerase sigma 32 holoenzyme. This 32-kD stress factor belongs to the sigma-70 factor family and is involved in intracellular temperature regulation, initiating bacterial transcription of various chaperone proteins and proteases in response to sudden increases in ambient temperature. The gene is currently classified as essential, due to the fact that rpoH deletion mutants fail to grow at standard incubation temperatures.

This part may be employed in heat shock-to-PoPs sensors by coupling expression of the rpoH gene to action of sigma 32-inducible promoters such as the htpG heat-shock promoter (part#: BBa_J45405). Transcriptional activation by rpoH transcripts is possible both within and without the sensor genetic circuit, as sigma factors can diffuse around the nucleus.

Note that this part is unsuitable for use by iGEM teams because it is incompatible with the BioBrick standard/RFC[10]. If you are an iGEM team wanting to use sigma 32 transcription factor please use BBa_K1895007 which removes the PstI site in this sequence.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 601
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 601
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 601
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 601
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 303
  Illegal SapI.rc site found at 652