Part:BBa_K1777003

miR-21 linear sponge

(β-globin SA)-(miR-21 binding site)6-(β-globin SD)
Function	Linear miR-21 sponge
Use in	Mammalian cells
RFC standard	RFC 10
Backbone	pSB1C3
Submitted by	[http://2015.igem.org/Team:Fudan Fudan 2015]

The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.
As the controls of blank groups, the linear miR-21 sponges have the similiar constructs with "Ouroboros"(Part:BBa_K1777016) and only lack inverted repeat sequences.

Figure 1: Construct design.

Figure 2: Examination of efficiency of miR-21 sponges.
At an appropriate molar ratio of luciferase tools and sponges,the expression of Renilla shall increase.

Proof of function

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 81
  Illegal XhoI site found at 452
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 423