Part:BBa_K1725080

Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site

Inverting regulatory region controlled by LacI (Part:BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong transcription that can nevertheless be repressed by LacI, the Lac repressor (BBa_C0012) (Lutz and Bujard, 1997) and induced by IPTG over a >600-fold range in E. Coli DH5-alpha-Z1 (Lutz and Bujard, 1997) and other lacI plus strains.

This part is identical to Part:BBa_R0011 except that an extra Nhe I site has been added to allow for easy checking for BioBrick insertion using a Nhe I restriction digest

Reference: Lutz R1, Bujard H. Nucleic Acids Res. 1997 25:1203-10. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]