Generator
eYFP_SH3 g

Part:BBa_K1602041

Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-09-17)

Inducible generator of eYFP with C-terminal SH3 ligand

This part is capable to generate the enhanced yellow fluorescent protein (eYFP) codon optimized for usage in E. coli (BBa_B0034) and under control of a T7-promotor. To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand construct was added. This ligand binds to the SH3 domain of the scaffold.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None