Coding
His-Scaffo

Part:BBa_K1602027

Designed by: Sebastian Barthel, Niklas Hummel, Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder   Group: iGEM15_TU_Darmstadt   (2015-09-16)

Protein scaffold with N-terminal HisTag

This part is capable of generating the whole protein scaffold containing the GBD,PDZ and SH3 domains. This scaffold was used by the iGEM Team TU Darmstadt 2015 to introduce a in vitro immobilized enzyme system. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 22
    Illegal BamHI site found at 877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 94
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None