Generator
Part:BBa_K1486017
Designed by: EPFL iGem team 2014 Group: iGEM14_EPF_Lausanne (2014-09-15)
fLuc[2]
This is the second part (C-terminal) of the split Firefly luciferase. To be used with fLuc[1] [[1]] for any split Firefly luciferase complementation assay.
Example of usage
We used this part in a complementation assay for the study of the interaction between CheY and CheZ bacterial chemotaxis protein (see here for details [[2]]).
Sequence and Features Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/light
//rbs/prokaryote/constitutive
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/light
//rbs/prokaryote/constitutive
biology | |
chassis | Escherichia coli |
color | Red |
direction | Forward |
emission | 550-620nm |
function | Reporter |
rbs | Elowitz |
resistance | Chloramphenicol |