Generator

Part:BBa_K1486016

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-15)

fLuc[1]

This is the first part (N-terminal) of the split Firefly luciferase. To be used with fLuc[2] [[1]] for any split Firefly luciferase complementation assay.

Example of usage

We used this part in a complementation assay for the study of the interaction between CheY and CheZ bacterial chemotaxis protein (see here for details [[2]]).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 135


[edit]
Categories
//cds/reporter
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/light
//rbs/prokaryote/constitutive
Parameters
biology
chassisEscherichia coli
colorRed
directionForward
emission550-620nm
functionReporter
rbsElowitz
resistanceChloramphenicol