Regulatory

Part:BBa_K1444003

Designed by: Dan Ziemianowicz   Group: iGEM14_Calgary   (2014-10-08)

Composite promoter and consensus B. subtilis RBS - C1-λ x3

A composite part consisting of the constitutive promoter Pveg BBa_K823003, followed by three copies of the repressible promoter C1-λ BBa_R0051, and ending with the consensus RBS from B. subtilis BBa_K090505.

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.

IMPORTANT NOTE: Our experience with this entire series of repressible promoters suggests that they are unstable in the standard biobrick vector pSB1C3. As such, they have been submitted to the registry in a pET28b+ vector BBa_K1444028.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None