Protein_Domain
SortA clv.

Part:BBa_K1362454

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

Sortase A recognition/cleavage site

This part codes for the amino acid sequence LPETGG. This site is recognized by Sortase A, which will cleave the Thr-Gly peptide bond and subsequently act as a transpeptidase to attach any peptide with an N-terminal glycine [1] [2]. The first four nucleotides of this sequence are CTTC, which will be the overhang produced wenn a forward-facing BsaI site (BBa_K1362424, BBa_K1362425) is directly following this part, as in the Sortase A circularization constructs (BBa_K1362202, BBa_K1362203, BBa_K1362204, BBa_K1362205).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None