Composite

Part:BBa_K1362205

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-07)


Sortase A non-circularization construct (with Smt3 and His6; without RBS)

TODO

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BamHI site found at 361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1174
    Illegal AgeI site found at 1286
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1463
    Illegal BsaI.rc site found at 389


[edit]
Categories
Parameters
None