RBS + NpuDnaE N-intein RFC assembly construct (with His6)
This intein assembly construct is part of the iGEM team Heidelberg 2014's strategy for cloning with split inteins. Inteins are naturally occuring peptide sequences that splice out of a precursor protein and attach the remaining ends together to form a new protein. When splitting this intein sequence into an N-terminal and a C-terminal split intein one is left with a powerful tool to post-translationally modify whole proteins on the amino-acid sequence level. This construct was designed to express any protein of interest fused to the Nostoc punctiforme DnaE N-terminal split intein. The corresponding C-terminal construct is BBa_K1362101. Upon coexpression or mixture of the N- and C-constructs protein splicing takes place and the N- and C-terminal proteins of interest are irreversibly assembled via a newly formed peptide bond.
This mechanism can be applied for a variety of different uses such as the activation of a protein through reconstitution of individually expressed split halves. See our split sfGFP experiment and the respective parts in the registry for more information. Protein splicing offers many new possibilities and we hope to have set a foundation that you guys can build on!
Usage and Biology
This Part is an N-intein assembly part according to RFC 105. Specifically, it contains a T7 Ribosome binding site, an mRFP selection marker enclosed by overhangs A and NN followed by the N-intein Sub-part for Npu DnaE N-Intein with a hexahistidine-tag fused to the C-terminus.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 826
Illegal AgeI site found at 938
- 1000Illegal BsaI site found at 1115
Illegal BsaI.rc site found at 40