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Experience: Works
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Translational_Unit
sfGFP_T65C

Part:BBa_K1362170

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer   Group: iGEM14_Heidelberg   (2014-10-09)

Split sfGFP reconstitution positive control

This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are BBa_K1362173 and BBa_K1362171, the N- and C-terminal non-splicing control halves are BBa_K1362174 and BBa_K1362172, respectively.

The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746908) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's wiki for a detailed documentation or find a summary of our results in the experience section of this part.

Usage and Biology

This

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 66


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