Split sfGFP reconstitution positive control
This part serves as positive control in a split sfGFP intein splicing assay. The N- and C-terminal splicing partners are BBa_K1362173 and BBa_K1362171, the N- and C-terminal non-splicing control halves are BBa_K1362174 and BBa_K1362172, respectively.
The assay was used to both characterize the reconstitution of split superfold GFP (BBa_I746908) as well as to demonstrate the splicing activity of the NpuDnaE split Intein BBa_K1362400. Visit the Heidelberg 2014's wiki for a detailed documentation or find a summary of our results in the experience section of this part.
Usage and Biology
Sequence and Features
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