Part:BBa_K1334005
P-formaldehyde+RBS+GFP+terminator
This part has been improved. The improved version is BBa_K2310901.
The promoter is activited by formaldehyde and the downstream GFP will be expressed.
Usage and Biology
We incubated E.coli strain DH5a with this part and stimulated the experimental group with 1mM formaldehyde when the OD600 value reached approximately one, which means E.coli comes into mid-log phase. And then we collected bacteria at certain time to measure the fluorescence and the absorbance.
From the Figure 1, we can see significant increase in relative fluorescence intensity after formaldehyde stimulation, which shows that our coloration system works well.
The Growth Condition curve in Figure 2 shows that the difference of the fluorescence/absorbance is not generated by the absorbance difference.
Figure 1 The function test of the formaldehyde promoter which shows our part BBa_K1334002 works well。
Figure 2 The growth condition of E.coli strain DH5a after formaldehyde stimulation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1265
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