Generator

Part:BBa_K2310901

Designed by: Yang Liang   Group: iGEM17_XMU-China   (2017-10-21)


T7 RNA polymerase generator controlled by formaldehyde

This part can be used to detect formaldehyde with a T7 promoter controlled reporter such as BBa_K2310002 or BBa_K2310003,

Usage and Biology

Compared with the existing formaldehyde detecting device, BBa_K1334005, the output signal produced by this part is stronger.

To test whether this part is better than the existing one, we transformed it into E.coli DH5α with BBa_K2310002, and measure the fluorescence intensity of GFP with 0.1mM formaldehyde.

XMU-2017-IF1.png

As the figure shows, this improved system has a stronger fluorescence intensity, about 1.4 times stronger than BBa_K1334005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None