Constitutive expression of GFP-REACh2 fusion protein
This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promoter. K1319002 is a dark quencher that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.
Usage and Biology
The two domains can be separated from each other via a TEV protease cleavage site in the linker.
More information about the characterization of this part can be found on the part page of K1319002.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1521
- 1000Illegal BsaI.rc site found at 706