Part:BBa_K1315012
Pseudomonas AutoInducer-1 response unit (luxR promoter)
This is a composite part that consists of three previously submitted biobricks. This is the business end of a PAI-1-activated biosensor from that uses the luxR promoter (BBa_R0079). It consists of a tet promoter from (BBa_R0040), which is constitutively on. This continually produces LasR (BBa_C0179). In the presence of the Pseudomonas AutoInducer 1 (PAI-1) molecule, LasR forms an complex with PAI-1 and it can then activate the luxR promoter (BBa_R0079), which leads to the production of GFP as a readout in a daughter device designed by iGEM Dundee 2014 (BBa_K1315010). This particular composite part has no reporter gene included so that it can be rapidly modified and tested by future teams.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 382
Illegal AgeI site found at 579 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 789
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