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Composite

Part:BBa_K1315011

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-27)

Pseudomonas AutoInducer-1 response unit (lasB promoter)

This is a composite part that consists of three previously submitted biobricks. This is the business end of a PAI-1-activated biosensor from that uses the lasB promoter (BBa_R0079). It consists of a tet promoter from (BBa_R0040), which is constitutively on. This continually produces LasR (BBa_C0179). In the presence of the Pseudomonas AutoInducer 1 (PAI-1) molecule, LasR forms an complex with PAI-1 and it can then activate the lasB promoter (BBa_R0079), which leads to the production of GFP as a readout in a daughter device designed by iGEM Dundee 2014 (BBa_K1315009). This particular composite part has no reporter gene included so that it can be rapidly modified and tested by future teams.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 849
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 579
  • 1000
    COMPATIBLE WITH RFC[1000]


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