Intermediate

Part:BBa_K1150062

Designed by: M. Agne, N. Gillich, A. Rudorf   Group: iGEM13_Freiburg   (2013-09-17)

NLS-BGH terminator

NLS-BGH
Function Nuclear localization and terminator

can be fused to (fusion) proteins

Use in Mammalians
RFC standard front RFC 25 & back RFC 10
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This intermediate part can be used for your own (fusion) proteins. With the nuclear localization sequence you make sure that your protein is transported into the nucleus in mammalian cells. The BGH terminator makes sure that transcription is terminated.


Usage

To assemble fusion proteins the beginning of this intermediate part is RFC25. The end is RFC 10.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Literature

[edit]
Categories
Parameters
None