Device

Part:BBa_K1150043

Designed by: Ilona Blank   Group: iGEM13_Freiburg   (2013-09-17)

uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3

pH1:tracrRNA pU6:DR_dummy_DR
Function two small RNAs giving rise to

specific binding of dCas9 to two VEGF loci

Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Streptococcus pyogenes
Source Feng Zhang, Addgene
Submitted by Freiburg 2013



Usage and Biology

By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the dCas9 derivates at once. The basis of the device is the RNAimer.
Here, we combined BBa_K1150037 and BBa_K1150038.


Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
    Illegal BglII site found at 1061
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 1053


Functional Parameters: Austin_UTexas

BBa_K1150043 parameters

Burden Imposed by this Part:

Burden Value: -1.2 ± 2.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

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Categories
Parameters
None