Regulatory

Part:BBa_K1114024

Designed by: Traci Haddock   Group: iGEM13_BostonU   (2013-09-12)

MoClo version of I13453 with FB fusion sites

This is the MoClo formatted part of BBa_I13453 with FB fusion sites. See the BBa_I13453 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM Distribution Kit


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Parameters
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