Plasmid

Part:BBa_K783055

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)

Level 0 MoClo Destination Vector with AB

This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 528


[edit]
Categories
Parameters
None