Regulatory
Part:BBa_K1114018
Designed by: Jake Awtry Group: iGEM13_BostonU (2013-09-07)
The MoClo format of BBa_J23116 with AB fusion sites.
This is the MoClo formatted part of BBa_J23116 with AB fusion sites. See the BBa_J23116 page for full information on the part.
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .
Usage and Biology
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
iGEM Distribution Kit
[edit]
Categories
Parameters
None |