Regulatory

Part:BBa_K1114016

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-07)

Owl Trial

Part Description

This is a promoter from the Anderson Promoter library in MoClo format. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Summary

Contact Information

Author(s)
Date
Team
Data Collection
Affiliation
Contact

Design Details

Type
Design Components
Vector
Additional Comments

Assembly Information

Assembly Method(s)
Assembly RFC
Scars
Assembly Components
Assembly Graph
Chassis
Strain
Additional Comments

Restriction Map

Flow Cytometry Experiment

BBa_K1114016 short This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM Distribution Kit


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Categories
Parameters
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