Designed by: Maithili Krishnan Group: iGEM13_TU-Delft (2013-09-20)
Lysis device with corrected Suffix
This part is an improvement of the Bio-brick Bba_K112808. It has a corrected suffix from the earlier version.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12INCOMPATIBLE WITH RFCIllegal NheI site found at 1350
Illegal NheI site found at 1373
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal AgeI site found at 955
Illegal AgeI site found at 1025
- 1000INCOMPATIBLE WITH RFCIllegal SapI site found at 1606
For more info, visit Killswitch on TU Delft iGEM13 Wiki
The construct BBa_K1022109 is made by ligating the existing biobrick BBa_K112808 in pSB1C3 backbone in order to correct the suffix, as it was observed that the suffix in the existing part (BBa_K112808) didnot have a Not I site.
This correction is useful for future cloning and sequence analysis. RFC10 standard is thus fixed due to this.
For a detailed characterization of the more improved part BBa_K1022114 with a pT7 promoter, please visit Killswitch on TU Delft iGEM13 Wiki.
|n/a||Lysis device with corrected Suffix|