Device

Part:BBa_K1022109

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-20)

Lysis device with corrected Suffix

This part is an improvement of the Bio-brick Bba_K112808. It has a corrected suffix from the earlier version.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1350
    Illegal NheI site found at 1373
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1025
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1606

Characterization

For more info, visit [http://2013.igem.org/Team:TU-Delft/Killswitch Killswitch] on TU Delft iGEM13 Wiki

Suffix Improvement

Introduction:

The construct BBa_K1022109 is made by ligating the existing biobrick BBa_K112808 in pSB1C3 backbone in order to correct the suffix, as it was observed that the suffix in the existing part (BBa_K112808) didnot have a Not I site.

This correction is useful for future cloning and sequence analysis. RFC10 standard is thus fixed due to this.

For a detailed characterization of the more improved part BBa_K1022114 with a pT7 promoter, please visit [http://2013.igem.org/Team:TU-Delft/Killswitch Killswitch] on TU Delft iGEM13 Wiki.


[edit]
Categories
Parameters
n/aLysis device with corrected Suffix