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Part:BBa_J70466
Designed by: Julie Norville Group: Knight Lab (2009-08-17)
yfp RBS, {0,5;15,10} family member - B0035 simulator (forward oligo)
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.
Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)
Also gtg starts are removed to make the RBS more predictable.
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} a tgagc ^ {rest of YFP (E0030)}
{promoter} at ^ gatct c {rbs master sequence} t^ actcg {rest of YFP (E0030)}
Forward oligo:
g {rbs master sequence} a tgagc
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} a)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |