Part:BBa_J70463

yfp RBS, {0,5;15,10} family member - B0033 simulator (reverse oligo)

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

Also gtg starts are removed to make the RBS more predictable.

Form of insert:

{promoter} ta ctaga ^ g {rbs master sequence} a tgagc ^ {rest of YFP (E0030)}

{promoter} at ^ gatct c {rbs master sequence} t^ actcg {rest of YFP (E0030)}

Forward oligo:

g {rbs master sequence} a tgagc

Reverse oligo:

Reverse complement of (ctagag {rbs master sequence} a)

Sequence and Features

Assembly Compatibility: