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Part:BBa_J70439

Designed by: Julie Norville   Group: Knight Lab   (2009-08-17)

yfp RBS, {0,5;15,10} family member - level 10 (master sequence)

These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-YFP to (Promoter X)-(RBS level Y)-YFP.

Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)

Also gtg starts are removed to make the RBS more predictable. 

Form of insert:

{promoter}  ta     ctaga ^ g            {rbs master sequence}           a  tgagc ^  {rest of YFP (E0030)}
{promoter}  at ^   gatct   c            {rbs master sequence}           t^ actcg     {rest of YFP (E0030)}

Forward oligo:
g {rbs master sequence} a tgagc

Reverse oligo
Reverse complement of (ctagag {rbs master sequence} a)


Usage and Biology

yfp RBS, {0,5;15,10} family member - level 10 (calculated 9.8)
Master sequence=BBa_J70439, forward oligo=BBa_J70440, or reverse oligo=BBa_J70441
yfp RBS, {0,5;15,10} family member - level 100 (calculated 110.5)
Master sequence=BBa_J70442, forward oligo=BBa_J70443, or reverse oligo=BBa_J70444
yfp RBS, {0,5;15,10} family member - level 1000 (calculated 1051.4)
Master sequence=BBa_J70445, forward oligo BBa_J70446, or reverse oligo BBa_J70447
yfp RBS, {0,5;15,10} family member - level 10000 (calculated 9096.9)
Master sequence=BBa_J70448, forward oligo=BBa_J70449, or reverse oligo=BBa_J70450
yfp RBS, {0,5;15,10} family member - level 100000 (calculated 107160.2)
Master sequence=BBa_J70451, forward oligo=BBa_J70452, or reverse oligo=BBa_J70453
yfp RBS, {0,5;15,10} family member - B0029 simulator (calculated 27.8)
Master sequence=BBa_B0029, forward oligo=BBa_J70454, or reverse oligo=BBa_J70455
yfp RBS, {0,5;15,10} family member - B0030 simulator (calculated 413.8)
Master sequence=BBa_B0030, forward oligo=BBa_J70456, or reverse oligo=BBa_J70457
yfp RBS, {0,5;15,10} family member - B0031 simulator (calculated 38.2)
Master sequence=BBa_B0031, forward oligo=BBa_J70458, or reverse oligo=BBa_J70459
yfp RBS, {0,5;15,10} family member - B0032 simulator (calculated 67.9)
Master sequence=BBa_B0032, forward oligo=BBa_J70460, or reverse oligo=BBa_J70461
yfp RBS, {0,5;15,10} family member - B0033 simulator (calculated 4.1)
Master sequence=BBa_B0033, forward oligo=BBa_J70462, or reverse oligo=BBa_J70463
yfp RBS, {0,5;15,10} family member - B0034 simulator (calculated 378)
Master sequence=BBa_B0034, forward oligo=BBa_J70464, or reverse oligo=BBa_J70465
yfp RBS, {0,5;15,10} family member - B0035 simulator (calculated 470)
Master sequence=BBa_B0035, forward oligo=BBa_J70466, or reverse oligo=BBa_J70467

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None