yeast GAL1 promoter
GAL1 promoter from S. cerevisiae Contains Kozak sequence, part BBa_J63003.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC
Team Czech Republic 2015 used this promoter very extensively. As a result, the characterization of this part was improved. See the Experience page for details.
JHU Igem 2011 is working on further characterization of this part through using GFP expression within the next month.
Team USTC 2016 modify this part to make it "ready to use". It is more convenient to be utilized now. See the Experience page for details.
Promoter dose =
Gal1 prevents growth in media containing galactose.
SCUT iGEM 2014 have designed a experiment to quantify Gal1 dose titer.
Characterization by iGEM Kyoto 2017
We used this promoter in order to express long hairpin RNA in yeast. Long hairpin RNA targeting B. xylophilus AK1 mRNA or GFP mRNA was cloned downstream of the promoter and introduced into the 2-micron high copy number plasmid of budding yeast, and subsequently expressed in yeast. GPD promoter ( BBa_K571001 ) was used as a control. For the data, please refer to the GPD Promoter page ( BBa_K571001 ) and our Wiki Result.
In order to kill pine wood nematodes by feeding RNAi, we used this part as a promoter to express hairpin-loop dsRNA in yeast. Then we confirmed the expression level of dsRNA in yeast by qRT-PCR and fed the yeast to pine wood nematodes.
We attached a hairpin loop sequence behind this part so that you can easily make hairpin type dsRNA targeted to your favorite gene. You can create a plasmid transcribing dsRNA with a hairpin loop which can be expressed in budding yeast by cleaving with the restriction enzyme NotI, connecting the sense part between the promoter and loop parts, then cleaving with the restriction enzyme HindIII for ligating the antisense part.