Composite

Part:BBa_J52022

Designed by: Matej Skocaj   Group: iGEM2006_Slovenia   (2006-10-14)


NfΚB-dnTraf6-linker-GFP

Part is composed of NfΚB promoter (recognation of this promoter for initiation of transcription depends on the presence of nuclear factor kappa B), TIR domain of Traf6 which is linked with 6 amino acid long linker to GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 776
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1459



Functional Parameters: Austin_UTexas

BBa_J52022 parameters

Burden Imposed by this Part:

Burden Value: 3.4 ± 4.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

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