Generator

Part:BBa_J45299

Designed by: André Green II   Group: iGEM2006_MIT   (2006-10-28)


PchA & PchB enzyme generator

This part is an intermediate to the protein generator BBa_45300.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
    Illegal NgoMIV site found at 732
    Illegal NgoMIV site found at 857
    Illegal NgoMIV site found at 868
    Illegal NgoMIV site found at 1149
    Illegal NgoMIV site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_J45299 parameters

Burden Imposed by this Part:

Burden Value: -2.7 ± 4.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

[edit]
Categories
//classic/generator/rct
Parameters
o_h
proteinPchA & PchB
rbs
tagNone