Part:BBa_J18905

pSB1AK3X Freiburg Fusion -> BioFusion conversion plasmid

pSB1AK3X shares the same backbone as pSB1AK3 but uses modified prefix and suffix sequences (that do not formally adhere to any BioBrick standard):

5' GAATTC GCGGCCGC T TCTAGA GCCGGC

  EcoRI    NotI     XbaI   NgoMIV

...part...

ACCGGT ACTAGT A GCGGCCG CTGCAG 3'

AgeI   SpeI     NotI    PstI

These prefix / suffix sequences are designed to translate protein parts from the proposed Fusion (aka Freiburg) format to the older BioFusion format from the Silver lab. Construction vectors with this modified Freiburg prefix / suffix could bring a protein part in frame with BioFusion parts and remove the STOP between the AgeI and SpeI site. Restriction / ligation with AgeI + NaeI can (theoretically) transfer Expression parts into this conversion vector which can then be used for normal BioFusion cloning (at the cost of adding a T G before and after the part). NaeI is an isoschizomer to NgoMIV but generates blunt ends which should allow for a directional transfer.

See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats BB formats].

pSB1AK3X is a high copy number plasmid carrying ampicillin and tetracyclin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

The given sequence starts with the modified suffix and ends with the modified prefix. The complete vector sequence is therefore obtained from: part + vector.