Part:BBa_J18902

pSB1AK3F construction plasmid for protein fusion BioBricks

pSB1AK3F is identical to pSB1AK3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix. This Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats BB formats].

pSB1AK3F is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.

The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 3177
  Illegal suffix found in sequence at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3177
  Illegal SpeI site found at 11
  Illegal PstI site found at 25
  Illegal NotI site found at 18
  Illegal NotI site found at 3183
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3177
  Illegal XhoI site found at 1044
  Illegal XhoI site found at 2070
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 3177
  Illegal suffix found in sequence at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 3177
  Illegal suffix found in sequence at 1
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2216