Reporter

Part:BBa_I739011

Designed by: Stefan Luzi   Group: iGEM07_ETHZ   (2007-10-18)

Double regulated expression cassette for GFP +LVA (I739107.B0034.J04031.B0015)

Part Structure

The Biobrick encodes LVA-tagged GFP (BBa_J04031) under control of the double promoter BBa_I739107 followed by the ribosome binding site BBa_B0034. The transcription of GFP is terminated by the double terminator BBa_B0015.

Mode of Action

Green fluorescent proteins (GFP) are fluorochromes, commonly used as reporter proteins.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is also termed Part 11.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 748


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Categories
Parameters
None