Part:BBa_I739004

Double regulated intermediate for P22 cII +LVA (I739102.B0034.C0053)

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) under control of the double promoter BBa_I739102 followed by the ribosome binding site BBa_B0034. Please notice that there is no terminator in this construct. If you plan to use this construct for double regulated P22 cII production, you should combine it with an appropriate terminator (e.g. BBa_B0015).

Mode of Action

P22 cII production can be repressed by cI and/or TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, TetR action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of cI.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is also termed Part 4. The synthesized P22 cII interacts with the double promoters BBa_I739104, BBa_I739103 and BBa_I739106 which are parts of composites BBa_I739006, BBa_I739016 and BBa_I739010 respectively. Together with Part 5, this Biobrick forms the composite part BBa_I739015.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 289