Intermediate

Part:BBa_I732074

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-08-02)

Promoter Adaptor + LacZ-alpha Reporter

This part, containing two gene fragments-Adaptor & LacZ alpha, which is added by the BioBrick Prefix and Suffix for standard assembly. The Promoter Adaptor is used for inserting certain parts without BioBrick Prefix or Suffix. The lacZ alpha is used for birthing reporter protein LACZ alpha for further assay.

Usage and Biology

  • The adaptor contains two restriction sites-XbaI and BglII because we ever used the promoters with the restriction site XbaI which is not a member in a standard biobrick prefix or suffix. So the adaptor is designed for this purpose.
  • lacZ alpha along with its RBS-B0034 is used as a reporter for promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None