Part:BBa_I13600

Tet with CFP reporter (without LVA tag)

The part glows (rather weakly) with a cyan colored flourescent protein. In the absence of the tetR protein, CFP expression is constitutive. tetR represses CFP production; this repression can be relieved by the addition of tetracycline or one of its [http://openwetware.org/wiki/ATc analogs (ie. aTc)].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Pictures

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  BBa_I13600 visualized under non-UV lightbox

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  BBa_I13600 visualized under 254nm wavelength UV

Functional Parameters: Austin_UTexas

BBa_I13600 parameters

Burden Imposed by this Part:

Burden Value: 3.0 ± 10.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.