Part:BBa_I13502

Screening plasmid intermediate

Built by Josh as an intermediate in screening plasmid construction.

[http://2018.igem.org/Team:Stockholm Team Stockholm 2018] improved this BioBrick by making it compatible with assembly standard RFC25 (see BioBrick: BBa_K2835000)
(iGEM Stockholm 2018 14.18, 29 September 2018 (UTC))

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 573
Illegal AgeI site found at 685
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_I13502 parameters

Burden Imposed by this Part:

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.