Reporter

Part:BBa_E0043

Designed by: Austin Che   Group: iGEM06_MIT   (2008-02-24)

green fluorescent protein (GFP) variant

This is intended to be a "better" GFP variant over BBa_E0040(GFPmut3). It is is closer to EGFP and contains half of the amino acid mutations present in Emerald. See http://openwetware.org/wiki/GFP for more information about GFP. Experimentally, it appears to be approximately the same or slightly brighter (~10%) than E0040.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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