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  • Plasmid backbones/Synthesis
    ==Plasmids used in DNA synthesis of BioBrick parts== ...t DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
    981 B (133 words) - 16:41, 22 December 2008
  • DNA Synthesis
    32 B (4 words) - 19:06, 9 June 2020

Page text matches

  • Part:BBa K419012:Experience
    ...indicates the CrtEBIY with constitutive promoter (R001) DNA fragment. The synthesis system composite was transformed into DH5-alpha E. coli for further experim ===Demonstration of Synthesis System [contains the part BBa_k419012 (constitutive promoter-CrtEBIY) in th
    5 KB (760 words) - 13:37, 6 November 2010
  • Part:BBa K3580102:Experience
    ==1. <i>in vivo</i> monoterpene synthesis== ...s.png|1500px|thumb|center|Fig. 1-1 Schematic of <i>in vivo</i> monoterpene synthesis]]
    13 KB (1,932 words) - 00:43, 3 December 2020
  • Chassis/Cell-Free Systems/Commercial E.coli S30/F2620
    #'''Rate of GFP synthesis''' of 100nM AHL #'''Transfer Function'''- [AHL] vs rate of GFP synthesis
    6 KB (1,037 words) - 00:37, 27 October 2007
  • Part:BBa K3580101:Experience
    ==1. <i>in vivo</i> monoterpene synthesis== ...s.png|1500px|thumb|center|Fig. 1-1 Schematic of <i>in vivo</i> monoterpene synthesis]]
    11 KB (1,666 words) - 00:44, 3 December 2020
  • Part:BBa K3711089
    ...er and blocks the expression of its downstream proteins, thus blocking the synthesis of pigment. ...her level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in natu
    12 KB (1,776 words) - 13:44, 21 October 2021
  • Part:BBa K4719017
    ...acterial cellulose-PHB polymer</b>. This was achieved introducing this PHB synthesis operon into <i> K. xylinus</i>. Therefore, after transformation with plasmi This construct is a polyhydroxybutyrate synthesis operon (<i>phaC, phaA, phaB</i>) producing PHB along with bacterial cellulo
    12 KB (1,760 words) - 22:09, 11 October 2023
  • Part:BBa K300010:Experience
    ...mated with the algorithm described in ''Data analysis to estimate the HSL synthesis rate per cell'' section. ...oach was proposed to estimate many interesting parameters, such as the HSL synthesis rate per cell, and an algorithm was proposed in order to evaluate the O.D.s
    28 KB (4,317 words) - 01:43, 28 October 2010
  • Part:BBa K300009:Experience
    ...estimated with the algorithm described ''Data analysis to estimate the HSL synthesis rate per cell'' section; ...oach was proposed to estimate many interesting parameters, such as the HSL synthesis rate per cell, and an algorithm was proposed in order to evaluate the O.D.s
    34 KB (5,311 words) - 03:18, 28 October 2010
  • Part:BBa I4102:Experience
    ...estimated with the algorithm described ''Data analysis to estimate the HSL synthesis rate per cell'' section; ...oach was proposed to estimate many interesting parameters, such as the HSL synthesis rate per cell, and an algorithm was proposed in order to evaluate the O.D.s
    35 KB (5,352 words) - 00:35, 28 October 2010
  • Part:BBa K3331012
    A circuit for efficient exopolysaccharide synthesis ...enzymes GalU and PmgA under the regulation of LacI-Ptrc promoter. The EPS synthesis circuit was as below:
    10 KB (1,547 words) - 11:04, 12 October 2023
  • Part:BBa K4182009
    ...er conservation and sand fixation of soil. We designed and constructed EPS synthesis circuit by overexpression of pgmA (phosphoglucose mutase)and galU (UDP gluc ...enzymes GalU and PmgA under the regulation of LacI-Ptrc promoter. The EPS synthesis circuit was as below:
    7 KB (982 words) - 04:10, 14 October 2022
  • Part:BBa K3633004
    ..._SFLS_SPBS--Indigo Synthesis--Pathway.png|600px|center|thumb|Fig 1. Indigo Synthesis Pathway]] ...l had lots of drawbacks that it can cause pollution and the substrates for synthesis were harmful to people's health.<br>
    8 KB (1,242 words) - 07:06, 4 October 2021
  • Part:BBa K3711088
    ...er and blocks the expression of its downstream proteins, thus blocking the synthesis of pigment. ...her level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in natu
    11 KB (1,611 words) - 13:43, 21 October 2021
  • Part:BBa K4182009:Design
    To facilitate the modularized design of plasmids, we named the EPS synthesis verification plasmid 4, which will be referred to as plasmid 4 in the follo ...n gene progenitor contains the EPS synthesis gene, LacI regulatory protein synthesis gene + Ptrc promoter, as shown in Figure 1.
    7 KB (1,089 words) - 05:57, 12 October 2022
  • Part:BBa K2365052
    === Origin: Saccharomyces cerevisiae, synthesis === Origin: synthesis
    14 KB (2,015 words) - 06:49, 20 October 2021
  • Part:BBa K2753002
    ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
    14 KB (2,217 words) - 03:24, 28 October 2020
  • Part:BBa K3711080
    ...her level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in natu ...arotenoids. The early steps of carotenoid biosynthesis pathway include the synthesis of Geranylgeranyl pyrophosphate (GGPP), the condensation of two molecules o
    9 KB (1,390 words) - 13:34, 21 October 2021
  • Part:BBa K3711083
    ...her level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in natu ...arotenoids. The early steps of carotenoid biosynthesis pathway include the synthesis of Geranylgeranyl pyrophosphate (GGPP), the condensation of two molecules o
    9 KB (1,383 words) - 13:39, 21 October 2021
  • Part:BBa K3711085
    ...her level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in natu ...arotenoids. The early steps of carotenoid biosynthesis pathway include the synthesis of Geranylgeranyl pyrophosphate (GGPP), the condensation of two molecules o
    9 KB (1,382 words) - 13:41, 21 October 2021
  • Part:BBa K3996014
    === Origin: Saccharomyces cerevisiae, synthesis === Origin: synthesis
    14 KB (1,932 words) - 16:53, 21 October 2021
  • Part:BBa K4595018
    ...eered bacterium <i> S.o oneidensis</i> MR-1, strengthening the common NAD+ synthesis pathway, and adding a new exogenous pathway for de novo NAD+ synthesis. Thus, the intracellular NAD+
    7 KB (1,109 words) - 03:02, 12 October 2023
  • Part:BBa K3932000:Design
    <li><b>The synthesis of Proteinase-K starts if our ECN senses arabinose by its pBAD promoter</b> ...ow ammonia level, activated NtrC induce the glnAp2 promoter to start TetR synthesis</li>
    20 KB (2,866 words) - 02:37, 22 October 2021
  • Part:BBa K4155002
    ...hesis method to synthesize zinc responsive promoter (pzntR) and riboflavin synthesis gene (ribB). ...lavin synthesis gene (ribB) by direct DNA synthesis method. The riboflavin synthesis gene for riboflavin expression promotes electron transfer from the cell to
    7 KB (1,056 words) - 08:27, 12 October 2022
  • Part:BBa F2620:Experience/Endy/Data analysis
    ...d, we can calculate the total synthesis rate of GFP and hence the per cell synthesis rate of GFP without considering dilution due to cell growth. ...r sec). We defined the output of <partinfo>BBa_E0240</partinfo> to be the synthesis rate of mature GFP, S_{cell} (GFP molecules per cell sec). The model inclu
    8 KB (1,329 words) - 17:16, 11 February 2008
  • Part:BBa K1349001
    <p>This part was designed to allow the rapid synthesis of the alarmone ppGpp, responsible for the stringent response in E. coli. I <p>When <em>Escherichia coli</em> cells are exposed to extreme starvation, synthesis of their rRNA is quickly curtailed for adapting the undernourished environm
    8 KB (1,297 words) - 22:12, 27 October 2020
  • Part:BBa K2587024
    ...R and then to the P<sub><i>lux</i></sub> promoter, which will activate the synthesis of the lysis protein E from the bacteriophage phiX174. ...ivity, which is necessary for catalysis of lipid I, a factor for cell wall synthesis (<strong>Cytolysis</strong>) (1) </li>
    10 KB (1,599 words) - 00:38, 18 October 2018
  • Part:BBa K3504015
    ...ex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is pro ...the identified interactions of nsP3 with host factors influence viral RNA synthesis or whether they are indicative of another nsP3 function that regulates the
    5 KB (713 words) - 20:35, 26 October 2020
  • Part:BBa K3633010
    ..._SFLS_SPBS--Indigo Synthesis--Pathway.png|600px|center|thumb|Fig 1. Indigo Synthesis Pathway]] ...l had lots of drawbacks that it can cause pollution and the substrates for synthesis were harmful to people's health.<br>
    4 KB (618 words) - 10:13, 27 October 2020
  • Part:BBa K3711090
    ...er and blocks the expression of its downstream proteins, thus blocking the synthesis of pigment. ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy
    10 KB (1,600 words) - 13:36, 21 October 2021
  • Part:BBa K3711091
    ...er and blocks the expression of its downstream proteins, thus blocking the synthesis of pigment. ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy
    10 KB (1,548 words) - 13:40, 21 October 2021
  • Part:BBa K4085015
    GDP-L-fucose is a key precursor for the synthesis of 2-FL, and it can be synthesized through two pathways. One is the de novo ...using the enzyme-catalyzed synthesis route, the yield of enzyme-catalyzed synthesis of 2'-FL is not high due to the expensive substrate guanosine 5’-diphosph
    16 KB (2,401 words) - 14:44, 12 October 2022
  • Part:BBa K4885006
    ...yl-CoA dehydrogenase are rate-limiting enzymes in the butyrate and butanol synthesis pathway. ...engineer C. tyrobutyricum to overexpressed several enzymes to enhance the synthesis pathway of butyrate and butanol. BBa_K4885002 were constructed to enhance t
    6 KB (828 words) - 13:29, 12 October 2023
  • Plasmid backbones/Synthesis
    ==Plasmids used in DNA synthesis of BioBrick parts== ...t DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
    981 B (133 words) - 16:41, 22 December 2008
  • Part:BBa K528004
    ===The mechanism of A/U-rich 5'-UTR to promote protein synthesis=== Fig.1. The mechanism of A/U-rich 5'-UTR to promote protein synthesis.<BR>
    4 KB (565 words) - 01:26, 22 October 2019
  • Part:BBa K554005
    Origin: synthesis ...We thus decided to create a completely new biobrick, responsible for Il-12 synthesis. The IL-12 gene is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil U
    8 KB (1,266 words) - 07:53, 6 October 2022
  • Part:BBa K759004
    Bacterial cellulose-PHB composite was achieved by introducing PHB synthesis operon into <i> K. xylinus</i>. This way, bacteria are able to simultaneous ...719017">BBa_K4719017</a>, containing <i>phaA</i>, is a polyhydroxybutyrate synthesis operon (<i>phaC, phaA, phaB</i>), producing PHB along with bacterial cellul
    4 KB (591 words) - 14:50, 12 October 2023
  • Part:BBa K2110105
    ...ethod in molecular biology. Production of proteins using cell-free protein synthesis usually takes a few hours, in contrast to production of proteins in cells, ...reeening of enzymes activities. In the post-genomic era, cell-free protein synthesis has rapidly become the preferred approach for high-throughput functional an
    6 KB (859 words) - 12:03, 20 October 2016
  • Part:BBa K1973012
    ...ve the implementation of a system that allows us to control externally the synthesis and turn-down of c-diGMP, we had cloned both genes pleD* and yhjH separatel ...of PleD* increases the intracellular concentration of c-diGMP, whereas the synthesis of YhjH decreases the intracellular concentration of this second messenger.
    10 KB (1,550 words) - 22:52, 19 October 2016
  • Part:BBa K1973023
    ...ve the implementation of a system that allows us to control externally the synthesis and turn-down of c-diGMP, we had cloned both genes pleD* and yhjH separatel ...of PleD* increases the intracellular concentration of c-diGMP, whereas the synthesis of YhjH decreases the intracellular concentration of this second messenger.
    10 KB (1,526 words) - 22:50, 19 October 2016
  • Part:BBa K274001
    ...s: League of Enhanced MicroGladiators." One of our methods of nanoparticle synthesis and attachment to cells is titled the recombinant method. Through the use o With this data, we intended to perform nanoparticle synthesis with L-tyrosine and MelA-transformed <i>E. coli</i> cells, and have these c
    11 KB (1,605 words) - 15:27, 12 October 2022
  • Part:BBa K2205002
    ...y designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. ...characteristics of an experiment, such as sensitivity, leakage, etc. DeGFP synthesis rate differs for the same element in different chassis. <br>
    6 KB (892 words) - 14:29, 21 October 2021
  • Part:BBa K2417000
    ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and showed some glucose oxidation activ ...eated with Ecotin-Proinsulin showed significantly higher rates of glycogen synthesis than those at basal levels, and a slight increase in glucose oxidation was
    7 KB (1,008 words) - 01:55, 1 November 2017
  • Part:BBa K2417001
    ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and also showed significantly greater g ...with Cytoplasmic-Proinsulin showed significantly higher rates of glycogen synthesis and glucose oxidation than basal levels. Significance calculated using an u
    6 KB (804 words) - 01:56, 1 November 2017
  • Part:BBa K2753015
    ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
    11 KB (1,720 words) - 08:21, 27 October 2020
  • Part:BBa K3022002
    .... We validated the function of KPD+CFPPK by performing intracellular polyP synthesis using phosphite as the substrate. ...P, +3 valance) as the solo phosphorus source was adopted to evaluate polyP synthesis.
    8 KB (1,161 words) - 13:32, 11 October 2022
  • Part:BBa K1807009
    Polyphosphate kinase (PPK) catalyzes the synthesis of polyphosphate (polyP). PolyP is a polyanionic biopolymer and generally s ...K. We validated the effectiveness of PPK-M on enhanced intracellular polyP synthesis by comparison with PPK.
    5 KB (695 words) - 12:18, 27 September 2022
  • Part:BBa K3504002
    ...ex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is pro ...the identified interactions of nsP3 with host factors influence viral RNA synthesis or whether they are indicative of another nsP3 function that regulates the
    7 KB (1,096 words) - 08:37, 21 October 2021
  • Part:BBa K3711081
    ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,337 words) - 13:38, 21 October 2021
  • Part:BBa K3711082
    ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,333 words) - 13:38, 21 October 2021
  • Part:BBa K3711084
    ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,329 words) - 13:41, 21 October 2021

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