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  • Collections/iGEM Type IIS Collections
    ==Team Uppsala 2020's iGEM Type IIS Standard collection== ...rted_Assembly_Standards”>registry standard</a></html>, and multiple Type IIS backbones and parts were present in the <html><a href=”https://parts.igem
    2 KB (321 words) - 17:22, 16 December 2020

Page text matches

  • Loop Assembly
    ...(Type IIS Golden Gate) standard, but we have not fully supported any Type IIS standard. That said, Type IIS assembly methods address some of the limitations of BioBricks while maintai
    9 KB (1,333 words) - 22:33, 30 November 2018
  • Part:BBa K2983030
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    16 KB (2,207 words) - 17:33, 21 October 2019
  • Part:BBa K2983031
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,123 words) - 18:16, 21 October 2019
  • Part:BBa K2983032
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:16, 21 October 2019
  • Part:BBa K2983033
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:17, 21 October 2019
  • Part:BBa K2983034
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:17, 21 October 2019
  • Part:BBa K2983035
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,121 words) - 18:18, 21 October 2019
  • Part:BBa K2983036
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983037
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983038
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983039
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:20, 21 October 2019
  • Collections/iGEM Type IIS Collections
    ==Team Uppsala 2020's iGEM Type IIS Standard collection== ...rted_Assembly_Standards”>registry standard</a></html>, and multiple Type IIS backbones and parts were present in the <html><a href=”https://parts.igem
    2 KB (321 words) - 17:22, 16 December 2020
  • Part:BBa K2983051
    ...pTef1 promoter that we'll refer to as pTef1d. It is derived from the wild type pTef1 promoter (nucleotides 1227374 to 1226969) of ''Yarrowia lipolytica'' ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
    15 KB (2,301 words) - 17:24, 21 October 2019
  • Part:BBa K2983053
    ...pTef1 promoter that we'll refer to as pTef1f. It is derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of ''Yarrowia lipolytica'' ...not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
    16 KB (2,353 words) - 17:21, 21 October 2019
  • Part:BBa K2983052
    ...not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard. ...how that this mutation, together with 4 others differences present in wild type as well as its shorter length, do not at all affect its activity when compa
    15 KB (2,294 words) - 17:15, 21 October 2019
  • Part:BBa K2983050
    ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt
    15 KB (2,282 words) - 17:23, 21 October 2019
  • Part:BBa K3122003
    ...he constitutive medium promoter, from Anderson family, was adapted to type IIS enzymes standard. <li><partinfo>BBa_K3122003</partinfo>: Promoter J23107 adapted to type IIS assembly</li>
    2 KB (308 words) - 09:42, 22 November 2019
  • Part:BBa K747000
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,956 words) - 00:13, 4 September 2020
  • Part:BBa K747005
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 19:02, 3 September 2020
  • Part:BBa K747002
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 20:04, 27 August 2020
  • Part:BBa K747001
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 22:26, 3 September 2020
  • Part:BBa K747003
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 22:23, 3 September 2020
  • Part:BBa K747004
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,929 words) - 04:07, 26 August 2020
  • Part:BBa K747007
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,933 words) - 02:37, 25 August 2020
  • Part:BBa K747008
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,959 words) - 00:05, 4 September 2020
  • Part:BBa K747009
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 22:29, 3 September 2020
  • Part:BBa K747010
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 18:56, 27 August 2020
  • Part:BBa K747011
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,933 words) - 03:39, 25 August 2020
  • Part:BBa K747012
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 21:03, 27 August 2020
  • Part:BBa K747019
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,938 words) - 20:32, 27 August 2020
  • Part:BBa K747018
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,938 words) - 03:31, 26 August 2020
  • Part:BBa K747020
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,939 words) - 04:15, 25 August 2020
  • Part:BBa K747021
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,935 words) - 19:04, 27 August 2020
  • Part:BBa K747022
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,936 words) - 02:50, 25 August 2020
  • Part:BBa K747023
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,935 words) - 18:45, 3 September 2020
  • Part:BBa K747047
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    11 KB (1,781 words) - 02:58, 18 October 2018
  • Part:BBa K3425017
    <p>This series of iGEM Type IIS templates present easy to use blueprints to design parts for this assembly ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a>.</p>
    4 KB (666 words) - 21:04, 25 October 2020
  • Part:BBa K3425018
    <p>This series of iGEM Type IIS templates present easy to use blueprints to design parts for this assembly ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a>.</p>
    4 KB (699 words) - 21:05, 25 October 2020
  • Part:BBa K3425020
    <p>This series of iGEM Type IIS templates present easy to use blueprints to design parts for this assembly ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a>.</p>
    5 KB (708 words) - 21:06, 25 October 2020
  • Part:BBa K2983074
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    15 KB (2,301 words) - 16:30, 21 October 2019
  • Part:BBa K2983174
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,317 words) - 21:07, 21 October 2019
  • Part:BBa K2983075
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,304 words) - 16:44, 21 October 2019
  • Part:BBa K2983175
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,317 words) - 21:06, 21 October 2019
  • Part:BBa K2983076
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,304 words) - 16:45, 21 October 2019
  • Part:BBa K2983176
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,317 words) - 21:05, 21 October 2019
  • Part:BBa K2983077
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,304 words) - 16:46, 21 October 2019
  • Part:BBa K2983177
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,317 words) - 21:05, 21 October 2019
  • Part:BBa K2983078
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,304 words) - 16:46, 21 October 2019
  • Part:BBa K2983178
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    16 KB (2,317 words) - 21:03, 21 October 2019
  • Part:BBa K3425019
    <p>This series of iGEM Type IIS templates present easy to use blueprints to design parts for this assembly ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a>.</p>
    4 KB (606 words) - 21:05, 25 October 2020
  • Part:BBa K3425033
    <a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">Type IIS iGEM Standard assembly</a> ...f="https://parts.igem.org/Collections/iGEM_Type_IIS_Collections">iGEM Type IIS standard collection</a>. Sequencing results of this part cloned in pSB1C00
    4 KB (711 words) - 12:19, 26 October 2020
  • Part:BBa K3425032
    ...for <a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">Type IIS iGEM Standard assembly</a> (RFC1000) by adding a five-nucleotide spacer (TA ...f="https://parts.igem.org/Collections/iGEM_Type_IIS_Collections">iGEM Type IIS standard collection</a>.</p>
    4 KB (658 words) - 21:24, 25 October 2020
  • Part:BBa K3425031
    <a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">Type IIS iGEM Standard assembly</a> ...f="https://parts.igem.org/Collections/iGEM_Type_IIS_Collections">iGEM Type IIS standard collection</a>. Sequencing results of this part cloned in pSB1C00
    4 KB (710 words) - 12:19, 26 October 2020
  • Part:BBa K2918012
    ...</a></html>. With that in mind, we, from TU Delft 2019, have designed Type IIS parts of both ribozymes and RBS for modular assembly in any combination des Unfortunately, junction sequences such as Type IIS overhangs between ribozyme and RBS can also influence translation rates. To
    8 KB (1,015 words) - 17:52, 6 December 2019
  • Part:BBa K4579007
    ...ssary to secrete small peptides using the <i>E. coli</i> microcin V (MccV) type I secretion system (T1SS) shown in Figure 2 of our <html><a href="https://2 ...omoter (Type 2), coding sequence (Type 3), and terminator/regulatory gene (Type 4) parts that can be easily assembled to express microcins either constitut
    9 KB (1,417 words) - 09:38, 12 October 2023
  • Part:BBa K3425009
    ...e pSB4K01-pSB4K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4K5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,130 words) - 20:51, 27 October 2020
  • Part:BBa K3425010
    ...e pSB4K01-pSB4K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4K5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,130 words) - 20:51, 27 October 2020
  • Part:BBa K3425011
    ...e pSB4K01-pSB4K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4K5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,130 words) - 21:18, 27 October 2020
  • Part:BBa K3425012
    ...e pSB4K01-pSB4K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4K5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,130 words) - 20:53, 27 October 2020
  • Part:BBa K3425013
    ...e pSB4A01-pSB4A04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4A5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,132 words) - 20:53, 27 October 2020
  • Part:BBa K3425014
    ...e pSB4A01-pSB4A04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4A5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,132 words) - 20:53, 27 October 2020
  • Part:BBa K3425015
    ...e pSB4A01-pSB4A04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4A5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,132 words) - 20:54, 27 October 2020
  • Part:BBa K3425016
    ...e pSB4A01-pSB4A04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB4A5 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,132 words) - 20:54, 27 October 2020
  • Part:BBa K4190008
    integration. GGA exploits type IIS restriction enzymes, which cleave DNA surrounding integration. All type II restriction enzymes leave “sticky ends” on the backbone and
    3 KB (395 words) - 01:22, 23 September 2022
  • Part:BBa K4190009
    integration. GGA exploits type IIS restriction enzymes, which cleave DNA surrounding integration. All type II restriction enzymes leave “sticky ends” on the backbone and
    3 KB (395 words) - 01:24, 23 September 2022
  • Part:BBa K3122000
    Our group have adapted this functional display system to type IIS enzymes grammar, and called it AEGIS's lamB display system for Gram-negativ <li><partinfo>BBa_K3122003</partinfo>: Promoter J23107 adapted to type IIS assembly</li>
    3 KB (424 words) - 00:47, 22 October 2019
  • Part:BBa K3122005
    <li><partinfo>BBa_K3122003</partinfo>: Promoter J23107 adapted to type IIS assembly</li> <li><partinfo>BBa_K2656009</partinfo>: RBS  B0030 adapted to type IIS assembly</li>
    6 KB (911 words) - 09:28, 22 November 2019
  • Part:BBa K3425021
    <p>Type IIS iGEM standard allows for fast and efficient construction of transcriptional ...on an existing Modular Assembly method [1]. These parts mimic a basic Type IIS part or a transcriptional unit and can be included in the assembly whenever
    4 KB (686 words) - 17:43, 26 October 2020
  • Part:BBa K3425022
    <p>Type IIS iGEM standard allows for fast and efficient construction of transcriptional ...on an existing Modular Assembly method [1]. These parts mimic a basic Type IIS part or a transcriptional unit and can be included in the assembly whenever
    4 KB (690 words) - 17:45, 26 October 2020
  • Part:BBa K3425001
    ===iGEM Type IIS Vectors=== ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    6 KB (963 words) - 12:35, 26 October 2020
  • Part:BBa K3425002
    ===iGEM Type IIS Vectors=== ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    6 KB (963 words) - 12:36, 26 October 2020
  • Part:BBa K3425003
    ===iGEM Type IIS Vectors=== ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    6 KB (963 words) - 12:37, 26 October 2020
  • Part:BBa K3425004
    ===iGEM Type IIS Vectors=== ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    6 KB (963 words) - 12:37, 26 October 2020
  • Part:BBa K3520002:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:25, 28 October 2020
  • Part:BBa K3520004:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:29, 28 October 2020
  • Part:BBa K3520031:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:16, 28 October 2020
  • Part:BBa K3520006:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:42, 28 October 2020
  • Part:BBa K3520005:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:39, 28 October 2020
  • Part:BBa K3520003:Design
    ==2. Elimination of restriction sites for illegal sites of TYPE IIS and RFC10 Assembly== ...sed, they will be inserted in the pHimarEm1 plasmid in one step using Type IIS assembly, in the designated order due to the 5’ and 3’ fusion sites.<br
    4 KB (630 words) - 00:23, 28 October 2020
  • Part:BBa K3781001
    ...by <b>Weber et al.</b> in <b>2011</b> and relies on the ability of <b>type IIS restriction enzymes</b> to cut DNA outside of their recognition sequence, h <p>The best part? Because of the type IIS restriction properties and the specifity of the generated overhangs, restri
    19 KB (3,101 words) - 22:50, 21 October 2021
  • Part:BBa K3781011
    ...by <b>Weber et al.</b> in <b>2011</b> and relies on the ability of <b>type IIS restriction enzymes</b> to cut DNA outside of their recognition sequence, h <p>The best part? Because of the type IIS restriction properties and the specifity of the generated overhangs, restri
    16 KB (2,527 words) - 21:51, 21 October 2021
  • Part:BBa K3781013
    ...by <b>Weber et al.</b> in <b>2011</b> and relies on the ability of <b>type IIS restriction enzymes</b> to cut DNA outside of their recognition sequence, h <p>The best part? Because of the type IIS restriction properties and the specifity of the generated overhangs, restri
    11 KB (1,710 words) - 08:09, 17 October 2021
  • Part:BBa K3781009
    ...by <b>Weber et al.</b> in <b>2011</b> and relies on the ability of <b>type IIS restriction enzymes</b> to cut DNA outside of their recognition sequence, h <p>The best part? Because of the type IIS restriction properties and the specifity of the generated overhangs, restri
    11 KB (1,808 words) - 08:08, 17 October 2021
  • Part:BBa K3781019
    ...by <b>Weber et al.</b> in <b>2011</b> and relies on the ability of <b>type IIS restriction enzymes</b> to cut DNA outside of their recognition sequence, h <p>The best part? Because of the type IIS restriction properties and the specifity of the generated overhangs, restri
    23 KB (3,756 words) - 21:09, 21 October 2021
  • Part:BBa K2117001
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    12 KB (1,839 words) - 16:05, 21 October 2019
  • Part:BBa K2117005
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    17 KB (2,541 words) - 18:52, 19 October 2021
  • Part:BBa K2983072
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,903 words) - 16:15, 21 October 2019
  • Part:BBa K2983170
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,915 words) - 00:00, 22 October 2019
  • Part:BBa K2983070
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,889 words) - 16:01, 21 October 2019
  • Part:BBa K2983071
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,903 words) - 16:11, 21 October 2019
  • Part:BBa K2983171
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,916 words) - 00:00, 22 October 2019
  • Part:BBa K2983172
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,916 words) - 23:59, 21 October 2019
  • Part:BBa K2983173
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,916 words) - 23:58, 21 October 2019
  • Part:BBa K2983073
    ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. We will refer to [[Part:BBa_K2983051|BBa_K2983051]] as
    13 KB (1,903 words) - 16:15, 21 October 2019
  • Part:BBa K1763444
    ...silkworm silk constructions ligated to a reporting marker flanked by Type IIS restriction sites was cloned and expressed in <i>E. coli</i> colonies. Prel ...ficiently functionalize silk fibers. In essence, we spin a mixture of wild-type silk dope spiked with a small volume of functional domain. This functional
    34 KB (4,992 words) - 03:39, 19 September 2015
  • Part:BBa K3425005
    ...e pSB3K01-pSB3K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB3K3 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,082 words) - 20:50, 27 October 2020
  • Part:BBa K3425006
    ...e pSB3K01-pSB3K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB3K3 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,082 words) - 20:50, 27 October 2020
  • Part:BBa K3425007
    ...e pSB3K01-pSB3K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB3K3 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,082 words) - 20:50, 27 October 2020
  • Part:BBa K3425008
    ...e pSB3K01-pSB3K04 series of plasmids is based on the pOdd set of iGEM Type IIS Vectors and are built upon the pSB3K3 plasmid backbone. When four Level 2 M ...href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">iGEM Type IIS standard guidebook</a></html>.
    13 KB (2,082 words) - 20:50, 27 October 2020
  • Part:BBa K2908671
    ...tion enzymes , called IIS cloning, namely ‘Golden Gate’ cloning. Type IIS restriction enzyme can specifically identify the target sites on double-str "Golden Gate" cloning method is in the same reaction system, using IIS type restriction endonuclease, cut beyond recognition site of DNA, DNA fragments
    4 KB (557 words) - 19:38, 21 October 2019

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