Part:BBa_T2013:Design

Strep-tag II head domain (WSHPQFEK)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This domain encodes the Strep-tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

An ATG start codon was used followed by a AAA second codon. AAA is the preferred codon for the second codon position in E. coli.

Source

This is a synthetic sequence.

References

<biblio>

1. Schmidt pmid=8433964
2. Skerra07 pmid=17571060
3. Skerra00 pmid=11036648
4. Skerra99 pmid=10796988
5. Skerra96 pmid=8636976

</biblio>